Virus:Article Title: A prefrontal cortex-lateral hypothalamus circuit controls stress-driven increased food intake.
Article Snippet: .. The pAAV-hSyn-DIO-hM4D(Gi)-mCherry (DREADD virus) (4*10^12 gc/ml, Addgene, United States, addgene.org) or pAAV-hSyn-DIO-mCherry (4.2*10^12 gc/ml, Addgene, United States, addgene.org) were bilaterally injected into the mPFC, (in mm: +1.8AP, -1.2ML from Bregma and, -2.3 DV, from skull, at a 10° angle) with an injection-rate of 100 nl/min, and a volume of 300 nl. .. The Cav2-cre-virus (2*10^12 gc/ml, Plateforme de Vectorologie de Montpellier, https://plateau-igmm.pvm.cnrs.fr/), combined with red-retrobeads (60x dilution, Lumafluor Inc, lumafluor.com) for injection site verification, was bilaterally injected into the anterior LHA (in mm: -1AP, 1.85ML, 5.36DV from Bregma, at a 10° angle), and posterior LHA (in mm: -1.85AP, 1.9ML, 5.41DV, from Bregma, at a 10° angle) with an injection-rate of 100nl/min.
Article Title: A prefrontal cortex-lateral hypothalamus circuit controls stress-driven increased food intake.
Article Snippet: .. The pAAV-hSyn-DIO-hM4D(Gi)-mCherry DREADD virus (300 nl 4*10^12 gc/ml, Addgene, United States) was injected in the LHA and to combined with the optogenetic optic fibers were implanted bilaterally in the LHA (-1.2 mm posterior to bregma; 1.85 mm lateral; -4.85 mm ventral from skull under an angle of 10°) and the light-sensitive Channelrhodopsin variant CoChR (300 nl rAAV5-Syn-CoChR-GFP at a titer of 3.8*10^12 gc/ml, UNC Vector Core) was injected bilaterally in the mPFC (+1.8mm anterior to bregma; 0.90 mm lateral; -2.2 mm ventral from skull under an angle of 10°). ..
Article Title: A prefrontal cortex-lateral hypothalamus circuit controls stress-driven food intake
Article Snippet: .. The pAAV-hSyn-DIO-hM4D(Gi)-mCherry (DREADD virus) (4*10^12 gc/ml, Addgene, United States, addgene.org ) or pAAV-hSyn-DIO-mCherry (4.2*10^12 gc/ml, Addgene, United States, addgene.org ) were bilaterally injected into the mPFC, (in mm: +1.8AP, -1.2ML from Bregma and, -2.3 DV, from skull, at a 10° angle) with an injection-rate of 100 nl/min, and a volume of 300 nl. .. The Cav2-cre-virus (2*10^12 gc/ml, Plateforme de Vectorologie de Montpellier, https://plateau-igmm.pvm.cnrs.fr/ ), combined with red-retrobeads (60x dilution, Lumafluor Inc, lumafluor.com ) for injection site verification, was bilaterally injected into the anterior LHA (in mm: -1AP, 1.85ML, 5.36DV from Bregma, at a 10° angle), and posterior LHA (in mm: -1.85AP, 1.9ML, 5.41DV, from Bregma, at a 10° angle) with an injection-rate of 100nl/min.
Injection:Article Title: A prefrontal cortex-lateral hypothalamus circuit controls stress-driven increased food intake.
Article Snippet: .. The pAAV-hSyn-DIO-hM4D(Gi)-mCherry (DREADD virus) (4*10^12 gc/ml, Addgene, United States, addgene.org) or pAAV-hSyn-DIO-mCherry (4.2*10^12 gc/ml, Addgene, United States, addgene.org) were bilaterally injected into the mPFC, (in mm: +1.8AP, -1.2ML from Bregma and, -2.3 DV, from skull, at a 10° angle) with an injection-rate of 100 nl/min, and a volume of 300 nl. .. The Cav2-cre-virus (2*10^12 gc/ml, Plateforme de Vectorologie de Montpellier, https://plateau-igmm.pvm.cnrs.fr/), combined with red-retrobeads (60x dilution, Lumafluor Inc, lumafluor.com) for injection site verification, was bilaterally injected into the anterior LHA (in mm: -1AP, 1.85ML, 5.36DV from Bregma, at a 10° angle), and posterior LHA (in mm: -1.85AP, 1.9ML, 5.41DV, from Bregma, at a 10° angle) with an injection-rate of 100nl/min.
Article Title: A prefrontal cortex-lateral hypothalamus circuit controls stress-driven increased food intake.
Article Snippet: .. The pAAV-hSyn-DIO-hM4D(Gi)-mCherry DREADD virus (300 nl 4*10^12 gc/ml, Addgene, United States) was injected in the LHA and to combined with the optogenetic optic fibers were implanted bilaterally in the LHA (-1.2 mm posterior to bregma; 1.85 mm lateral; -4.85 mm ventral from skull under an angle of 10°) and the light-sensitive Channelrhodopsin variant CoChR (300 nl rAAV5-Syn-CoChR-GFP at a titer of 3.8*10^12 gc/ml, UNC Vector Core) was injected bilaterally in the mPFC (+1.8mm anterior to bregma; 0.90 mm lateral; -2.2 mm ventral from skull under an angle of 10°). ..
Article Title: A prefrontal cortex-lateral hypothalamus circuit controls stress-driven food intake
Article Snippet: .. The pAAV-hSyn-DIO-hM4D(Gi)-mCherry (DREADD virus) (4*10^12 gc/ml, Addgene, United States, addgene.org ) or pAAV-hSyn-DIO-mCherry (4.2*10^12 gc/ml, Addgene, United States, addgene.org ) were bilaterally injected into the mPFC, (in mm: +1.8AP, -1.2ML from Bregma and, -2.3 DV, from skull, at a 10° angle) with an injection-rate of 100 nl/min, and a volume of 300 nl. .. The Cav2-cre-virus (2*10^12 gc/ml, Plateforme de Vectorologie de Montpellier, https://plateau-igmm.pvm.cnrs.fr/ ), combined with red-retrobeads (60x dilution, Lumafluor Inc, lumafluor.com ) for injection site verification, was bilaterally injected into the anterior LHA (in mm: -1AP, 1.85ML, 5.36DV from Bregma, at a 10° angle), and posterior LHA (in mm: -1.85AP, 1.9ML, 5.41DV, from Bregma, at a 10° angle) with an injection-rate of 100nl/min.
Variant Assay:Article Title: A prefrontal cortex-lateral hypothalamus circuit controls stress-driven increased food intake.
Article Snippet: .. The pAAV-hSyn-DIO-hM4D(Gi)-mCherry DREADD virus (300 nl 4*10^12 gc/ml, Addgene, United States) was injected in the LHA and to combined with the optogenetic optic fibers were implanted bilaterally in the LHA (-1.2 mm posterior to bregma; 1.85 mm lateral; -4.85 mm ventral from skull under an angle of 10°) and the light-sensitive Channelrhodopsin variant CoChR (300 nl rAAV5-Syn-CoChR-GFP at a titer of 3.8*10^12 gc/ml, UNC Vector Core) was injected bilaterally in the mPFC (+1.8mm anterior to bregma; 0.90 mm lateral; -2.2 mm ventral from skull under an angle of 10°). ..
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